Abstract
The kinetic method collects data throughout the course of the binding reaction and yields graphic estimations of k sub(a) and k sub(d), rate constants for association and dissociation, which provide K sub(A)(k sub(a)/k sub(d)), but, more importantly, also provide insight to the progress of the binding reaction. Other advantages to the kinetic approach include there is no assumption about single-step mechanisms, and there is no requirement for reversibility. The kinetic approach allows investigators to identify any departure from ideal pseudo-first-order attachment kinetics which can help identify the cell structures responsible for attachment. Kinetic analysis also allows the separate investigation of low-affinity (long-range) association and stereospecific binding occurring at a short range. Systems in which the energy barrier between the long-range attachment and closer, stronger binding is large benefit greatly from an analysis of the first, reversible stage of adhesion, because this is the most influential determinant of eventual adhesion. This stage is best studied kinetically.