Abstract
Pseudomonas fluorescens H2 was subjected to mutagenesis with Tn5, and of 1005 transconjugants, twelve had altered adhesion ability. Five attached in higher numbers to polystyrene test surfaces than the parent, and seven attached in lower numbers. PAGE analysis of outer membrane proteins and lipopolysaccharides demonstrated that all mutants were somewhat different; however, profiles of all mutants were more similar to each other than they were to those of H2. The results indicate that Tn5 insertion has resulted not only in altered adhesion ability but also in pleiotrophic phenotypic alterations in cell surface constituents.