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A Cell-Based Fluorescent Assay to Detect the Activity of AB Toxins that Inhibit Protein Synthesis: Methods and Protocols
Book chapter

A Cell-Based Fluorescent Assay to Detect the Activity of AB Toxins that Inhibit Protein Synthesis: Methods and Protocols

Patrick Cherubin, Beatriz Quinones, Salem Elkahoui, Wallace Yokoyama and Ken Teter
pp.25-36
Methods in Molecular Biology, Humana Press Inc
01/01/2017
DOI: https://doi.org/10.1007/978-1-4939-6958-6_3
PMID: 28478554

Abstract

Biochemical Research Methods Biochemistry & Molecular Biology Life Sciences & Biomedicine Microbiology Science & Technology
Many AB toxins elicit a cytotoxic effect involving the inhibition of protein synthesis. In this chapter, we describe a simple cell-based fluorescent assay to detect and quantify the inhibition of protein synthesis. The assay can also identify and characterize toxin inhibitors.

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