Abstract
For high frequency clonal propagation of Vanda teres, nodal segments are cultured oil VW medium supplemented with 2% sucrose, 2 mg/L Kinetin, 0.5 mg/L NAA, 2 g/L peptone, 1 g/L activated char-coal and 2.2 g/L gelrite. The Cultures are incubated at 24 +/- 2 degrees C under fluorescent light 50 mu mol/m(2)/s for a 16 h photoperiod per day. The PLBs (protocorm like bodies) arc induced within 12 weeks Of Culture and are subcultured to proliferate oil the fresh nutrient Culture medium for 8 weeks. The clumps of the PLBs are dissected and Cultured oil VW medium containing 2% sucrose, 15% Coconut water (CW), 2 g/L peptone, 150 mg/L 1-glutamine and I g/L activated charcoal. The PLB sections elongate to form shoots, and new PLBs arc induced from the base within 8 weeks Of Culture. For plantlets formation, the shoots are cultured oil VW medium amended with 2% sucrose, 15% CW, 2 g/L peptone, I g/L activated charcoal, 50 g/L banana pulp and I mg/L Indole-3-butyric acid (IBA). The regenerated plants are acclimatized and Cultivated in the nursery, where they bloom within 3 years.