MutRenSeq: A Method for Rapid Cloning of Plant Disease Resistance Genes

Burkhard Steuernagel; Kamil Witek; Jonathan D. G. Jones; Brande B. H. Wulff

doi:10.1007/978-1-4939-7249-4_19

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Book chapter

MutRenSeq: A Method for Rapid Cloning of Plant Disease Resistance Genes

Burkhard Steuernagel, Kamil Witek, Jonathan D. G. Jones and Brande B. H. Wulff

Methods in molecular biology (Clifton, N.J.), pp.215-229

Methods in Molecular Biology, Humana Press Inc

01/01/2017

DOI: https://doi.org/10.1007/978-1-4939-7249-4_19

PMID: 28856654

Abstract

Biochemical Research Methods

Biochemistry & Molecular Biology

Life Sciences & Biomedicine

Plant Sciences

Science & Technology

MutRenSeq is a method to clone disease resistance (R) genes in plants. Tips and detailed experimental protocols for the pipeline, including the complexity reduction by R gene targeted enrichment sequencing, and computational analysis based on comparative genomics are provided in this chapter.

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Title: MutRenSeq: A Method for Rapid Cloning of Plant Disease Resistance Genes
Creators - without role: Burkhard Steuernagel - Norwich Research Park
Kamil Witek - Sainsbury Laboratory
Jonathan D. G. Jones - Sainsbury Laboratory
Brande B. H. Wulff - Norwich Research Park
Contributors - without role: S Periyannan
Publication Details: Methods in molecular biology (Clifton, N.J.), pp.215-229
Series: Methods in Molecular Biology
Publisher: Humana Press Inc; TOTOWA
Number of pages: 15
Grant note: BB/L011794/1 / BBSRC; UK Research & Innovation (UKRI); Biotechnology and Biological Sciences Research Council (BBSRC) BB/L011794/1; BB/L009293/1; BB/H019820/1 / Biotechnology and Biological Sciences Research Council; UK Research & Innovation (UKRI); Biotechnology and Biological Sciences Research Council (BBSRC)
Identifiers: 9943744408331
Academic Unit: King Abdullah University of Science & Technology
Language: English
Resource Type: Book chapter