Abstract
In the last decades, the optical biosensors have great attention, especially, when the biosensor is inserted into a good host such as tetraethoxyorthosilicate (TEOS) polymer and molecular imprinted polymer with no interference. This method gives a transparent nano-optical biosensor with new optical properties to increase the impact of the proposed analytical method which has wide linear range and very low detection limit. In this chapter, the transparent nano-optical sensor is inserted into TEOS polymer and imprinted template in molecular imprinted nanopolymer for the determination of different food contaminants such as different bacteria (Salmonella, Staphylococcus aureus and Campylobacter), Aflatoxin produced by different Aspergillus flavus and Aspergillus parasiticus species of fungi and E. coli bacteria. The nano-optical biosensors were used for the determination of food contaminants in different food samples with a high-performance analytical method.