Abstract
Several methods have been developed from time and time for ensuring the facile preparation of organic nanoparticles. The sizes of nanoparticles depend on several parameters,
viz. amphiphilicity and molecular weight of the discrete organic molecules, the initial concentrations of organic molecule containing solutions, and the miscibility of organic solvents containing organic molecules with aqueous media. Basically, four important methods including self-assembly, polymerization, emulsification and nanoprecipitation/reprecipitation have been used for the preparation of FONs with diverse applications in analytical and biomedical sciences. Out of these techniques, nanoprecipitation is the simplest and the most widely used technique. This technique enables transformation of soluble organic molecules into nanoparticles in the aqueous media and later ensures their fast screening for various analytical and biomedical applications. In this chapter, attempts have been made to highlight the above four methods of the preparation of FONs. The discussion of these methods may help the readers in choosing their method for the preparation of new FONs with interesting properties.