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Quantification of Phytohormones by HPLC-MS/MS Including Phytoplasma-Infected Plants: Methods and Protocols
Book chapter

Quantification of Phytohormones by HPLC-MS/MS Including Phytoplasma-Infected Plants: Methods and Protocols

Marilia Almeida-Trapp and Axel Mithoefer
pp.345-358
Methods in Molecular Biology, Humana Press Inc
01/01/2019
PMID: 30362016

Abstract

Biochemical Research Methods Biochemistry & Molecular Biology Life Sciences & Biomedicine Microbiology Plant Sciences Science & Technology
There is strong evidence that phytohormones such as abscisic acid, auxin, salicylic acid, and jasmonates might play a role in defense of the host plants during phytoplasma infections. However, these compounds are usually present at low concentration in complex matrixes, requiring a sensitive and selective method to analyze and quantify them. Here, we present a HPLC-MS/MS method to quantify phytohormones in different infected and noninfected plant tissues.

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