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Single-Cell Quantitative PCR: Advances and Potential in Cancer Diagnostics
Book chapter

Single-Cell Quantitative PCR: Advances and Potential in Cancer Diagnostics

Chi Young Ok, Rajesh R. Singh and Alaa A. Salim
Clinical Applications of PCR, pp.153-159
Methods in Molecular Biology, Humana Press Inc
01/01/2016
PMID: 26843054

Abstract

Biochemical Research Methods Biochemistry & Molecular Biology Cell Biology Life Sciences & Biomedicine Science & Technology
Tissues are heterogeneous in their components. If cells of interest are a minor population of collected tissue, it would be difficult to obtain genetic or genomic information of the interested cell population with conventional genomic DNA extraction from the collected tissue. Single-cell DNA analysis is important in the analysis of genetics of cell clonality, genetic anticipation, and single-cell DNA polymorphisms. Single-cell PCR using Single Cell Ampligrid/GeXP platform is described in this chapter.

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