Abstract
The Cell Culture Hydrocyclone Perfusion System separates cells by hydrodynamic-driven centrifugal forces. Cells are only exposed for a very short period of time of less than 0.2 s during the process of separation. The small 10 cm high Cell Culture Hydrocyclone has a maximum process-specific perfusion performance of 450-720 liters of cell culture harvest per day while operating at a flow rate of 0.3-0.5 L min(-1). Its special properties - characterized by its small dimensions, in situ sterilisability, robustness, reusability, and simple adjustment for higher process volumes - emphasize the advantages that distinguish the Cell Culture Hydrocyclone from other cell separation systems.