Abstract
Semiconductor quantum dots arc recognized to provide a particularly effective approach to bright nano-objects for bioimaging. However, these inorganic systems suffer from several drawbacks such as toxicity, dispersity, blinking... and raise a number of questions with respect to environmental issues. With this in mind, we have developed an innovative route towards purely organic nanodots showing exceptional one and two-photon brightness by confining a large number of optimized fluorophores within nano-objects of defined and controlled structure. These novel "soft" nano-objects offer major promises for bio and nanophotonics.