Abstract
We are presenting cassette as a novel point of care diagnostic device. This device is easy to use, low cost to prepare, high throughput and can analyze several samples at the same time. We first, demonstrate the preparation method of the device. Then, fabrication of the flexible substrate has been presented. The device has been used for detection of the real sample of E.coli bacteria following by colorimetric detection. We have shown that we could detect 30 cfu/ml bacteria and 100 fg/mu l of Staphylococous aureus DNA in 1 hr using LAMP amplification technique. This device will be helpful in hospitals and doctor's office for analysis of several patients' samples at the same time.