Comparative Analysis of Genetic Diversity in Tunisian Collections of Date-Palm Cultivars Based on Random Amplified Polymorphic DNA and Inter Simple Sequence Repeats Fingerprints

S. Zehdi-Azouzi; S. Rhouma; A. Ould Mohamed Salem; H. Sakka; A. Rhouma; M. Marrakchi; M. Trifi

doi:10.17660/ActaHortic.2009.814.18

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Comparative Analysis of Genetic Diversity in Tunisian Collections of Date-Palm Cultivars Based on Random Amplified Polymorphic DNA and Inter Simple Sequence Repeats Fingerprints

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Comparative Analysis of Genetic Diversity in Tunisian Collections of Date-Palm Cultivars Based on Random Amplified Polymorphic DNA and Inter Simple Sequence Repeats Fingerprints

S. Zehdi-Azouzi, S. Rhouma, A. Ould Mohamed Salem, H. Sakka, A. Rhouma, M. Marrakchi and M. Trifi

XII EUCARPIA SYMPOSIUM ON FRUIT BREEDING AND GENETICS, Vol.814(814), pp.149-156

Acta Horticulturae

01/01/2009

DOI: https://doi.org/10.17660/ActaHortic.2009.814.18

Abstract

Agriculture

Horticulture

Life Sciences & Biomedicine

Plant Sciences

Science & Technology

This study characterized the genetic diversity of 17 Tunisian date-palm cultivars sampled from the Tozeur oasis using random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR). Both random and ISSR primers have generated a total of 54 RAPDs and 48 ISSRs. The derived genetic distances values suggested high level of diversity among the date-palm cultivars studied. In addition, data proved that in the Tunisian germplasm, information provided by RAPD and ISSR is not equivalent. The distribution of each class of markers in the genome can be a critical factor to explain this result.

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Title: Comparative Analysis of Genetic Diversity in Tunisian Collections of Date-Palm Cultivars Based on Random Amplified Polymorphic DNA and Inter Simple Sequence Repeats Fingerprints
Creators - without role: S. Zehdi-Azouzi - Fac Sci Tunis, Lab Genet Mol Immunol & Biotechnol, Campus Univ, El Manar Tunis 2092, Tunisia
S. Rhouma - Fac Sci Tunis, Lab Genet Mol Immunol & Biotechnol, Campus Univ, El Manar Tunis 2092, Tunisia
A. Ould Mohamed Salem - Univ Nouakchott, Fac Sci & Tech, Nouakchott, Mauritania
H. Sakka - Fac Sci Tunis, Lab Genet Mol Immunol & Biotechnol, Campus Univ, El Manar Tunis 2092, Tunisia
A. Rhouma - IPGRI, Ctr Rech Phoenicicoles, Degache 2260, Tunisia
M. Marrakchi - Fac Sci Tunis, Lab Genet Mol Immunol & Biotechnol, Campus Univ, El Manar Tunis 2092, Tunisia
M. Trifi - Fac Sci Tunis, Lab Genet Mol Immunol & Biotechnol, Campus Univ, El Manar Tunis 2092, Tunisia
Contributors - without role: M T Espiau
J M Alonso
Publication Details: XII EUCARPIA SYMPOSIUM ON FRUIT BREEDING AND GENETICS, Vol.814(814), pp.149-156
Series: Acta Horticulturae
Publisher: Int Soc Horticultural Science
Number of pages: 8
Grant note: Tunisian "Ministere de l'Enseignement Superieur, de la Recherche Scientifique et de la Technologie" "FEM-PNUD-IPGRI, RAB 98 G31" / IPGRI Project
Identifiers: 9922835308331
Academic Unit: King Khalid University
Language: English
Resource Type: Conference proceeding