Abstract
Mastitis diagnosis in sub clinical cases of cattle and buffalo especially in the Last trimester of the pregnancy has a prime importance and has great value regarding the control and treatment of the mastitic animals in time. For the diagnosis of mastitis 130 animals cows and buffalo cows were divided in to three groups, Group A, Group B and group C randomly and for sub clinical mastitis in cows and buffalo cows 20 animals of group B in Last trimester of pregnancy specially in the early 8(th) month were selected randomly. The animals were physically normal and their udder was normal and developing. Their 1 ml of colostrum was squeezed and mixed in the Petri-dish with equal volume of solution containing sodium carbonate and benzene sulfonic acid dissolved in distilled water. The test could compete the California Mastitis Test (CMT). This test could be the cheapest and easily made by the farmers and field veterinarians for the early diagnosis of the mastitis in pregnant and Lactating mastitic animals.