Abstract
We used zebrafish as the model animal and microinjected exogenous gene p204 into its zygotes. By observing the expression of the report gene GFP, we found that the expression of p204 in the embryo of zebrafish started from stage 8hpf, especially concentrating in the chest and stomach. Subsequently, p204 was highly expressed during stage 8hpf to 8dpf and reduced from 8dpf to about 11dpf until fluorescence completely disappeared. The injection of p204-EGFP could induce the change of phenotype of zebrafish, and cause deformity such as bigger abdomen and curly tail. Selecting the injected embryos which can express green fluorescence proteins to perform quality real time PCR to test the genes tbk1 and sting related to innate immunity, we discovered that the expressions of tbk1 and sting was apparently up regulated after the injection of p204 vector compared to the controls during the stage 8hpf-48hpf, but it showed no changes in stage 4hpf. In addition, we performed RNA in situ hybridization of relevant genes, and the results were identical with those by real time PCR, which suggest that: 1) the exogenous gene p204 can influence the development of zebrafish and cause abnormal phenotypes to a certain extent; 2) p204 can affect the innate immunity system of zebrafish and participate in the signaling pathways.