Abstract
The objective of this study was to determine the influence of sperm parameters on the rate of survival spermatozoa, 24 hours after sperm selection procedure. Routine semen analysis and DGC (density gradient centrifugation) procedure were performed in 85 couples undergoing ART (Assisted Reproductive Techniques). The number of motile spermatozoa recovered after DGC and the percentage of motile and viable spermatozoa present 24 hours after DGC procedure, were recorded. We used linear regression to study the relationship between the different sperm variables before and after CGD to the sperm survival after 24 hour incubation. We found that, among all analysed semen parameters, the percentage of sperm with excess residual cytoplasm and sperm flagellum anomalies determined after CGD procedure, had a negative influence on spermatozoa survival 24 hours after sperm selection (p < 0.01). This result suggests that immature spermatozoa induce sperm survival decrease by producing reactive oxygen species (ROS). Consequently, it's recommended to more consider in the future these sperm anomalies in the prediction of IVF outcome.