Abstract
High hydrogen yield was carried out using Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564). The ability of this Clostridium on hydrogen production was studied in 250 mL batch culture with glucose as the sole organic carbon source. The effect of initial substrate concentration, initial medium pH, addition of Fe(2+) and temperature were investigated. Results show that the highest yield (Y(p/s)) of hydrogen produced was about 3.10 mol (mol glucose)(-1) when the initial glucose concentration was 10 gL(-1), initial pH of 6.0 +/- 0.2 and temperature 37 degrees C. The yield of hydrogen produced decreased when higher initial glucose concentration was applied. The yield of hydrogen was increased when 25 mg L(-1) Fe(2+) was added to the medium.