Abstract
Marine-derived actinomycete
Nocardiopsis
sp. CMB-M0232 produces nocardiopsins (
1
-
2
), nocardioazines (
3
-
4
), and nocardiopyrone A, as first reported by Dr. Robert Capon's group (U. Queensland). Nocardiopsins (
1
-
2
) are the newest members of the rapamycin family of FKBP-modulatory natural products and the first family members featuring a tetrahyrdofuran moiety. Nocardioazines (
3
-
4
) are the only known C-3 normal prenylated diketopiperazines, and
3
inhibits p-glycoprotein to reverse drug resistance among cancer cells. This presentation will discuss the genetic and enzymatic basis for
Nocardiopsis
sp. natural product biosynthesis, as elucidated via
in vitro
enzyme assays, heterologous pathway expression, and metabolite profiling.
Fig. 1