Abstract
The success rate of the cryopreserved semen is very high for domestic animals compared to that of endangered animals. The aim of this study was to investigate the proper methods of semen dilution and to determine the efficiency of the cryopreservation efficiency of the semen collected from the male Arabian sand Ggazelles Males (Gazella subgutturosa marica).
Semen samples were collected monthly for a duration of one year from 10ten adult male Arabian sand gazelles using the electro-ejaculation method. For cryopreservation, the following two semen diluents were used: Triladyl and Tris-citrate. For live sperm, dead sperm, and sperm abnormalities, two staining methods and SEM were used. When the average of individual motility before freezing was compared with that after thawing, it was found that a significant number of spermatozoa (P<0.01) was adversely affected by freezing. It is concluded that the breeding season of Arabian sand gazelles males were in October and November.
The best cryopreservation results were obtained when the Triladyl extender was used rather than Tris diluents.