Abstract
In the recent years, many HLA laboratories predict crossmatch results based on the strength of Donor Specific Antibodies (DSA) measured by single antigen (SA) solid phase assays. This study evaluates the prediction of CDC crossmatch results to flow cytometry Median Channel Sshift (MCS) versus SA cumulative Median Fluorescent Intensity (MFI) of DSA.
93 T cell AHG-CDC and flow cytometry crossmatch (FCXM) were reviewed, results compared to DSA MFI (0-10000) (>10000). In the other hand, the same samples were compared to MCS (0-250)& (>250). Percentage frequency, chi-square and P-value were calculated to assess the association between mentioned variables.
DSA MFI’s and MCS were grouped into two groups (0-10000), (>10000), (0-250) & (>250) respectively and are presented in this table.[Table 1]
From the above data there was a significant association in prediction of positive CDC crossmatch based on MCS (chi square of 67.95, P<.0001) compared to SA cumulative MFI (Chi square 11.38, P=0.0007. The positive predictive value of the CDC crossmatch based on MCS was higher than MFI with 88% and 57% respectively. Therefore SA cumulative MFI is not significant to consider as predictor for a positive CDC crossmatch rather than transplant risk factor.