Abstract
In order to assess the effect of silica gel structure on retention in hydrophilic interaction chromatography, a test system was developed which used quaternary ammonium ions as probes with tetramethylammonium acetate (TMAA) as the counter-ion competing against the interaction of the test probes with ionised silanols in the stationary phase. Four silica gel columns and a silica hydride column were examined. Retention times were obtained for the test probes at 20, 40, 60, 80 and 90 % acetonitrile (ACN) with all the mobile phase mixtures containing 10-mM TMAA buffer at pH 6.0. All phases gave "U"-shaped plots for log k against percentage of ACN with the steepest rise in retention occurring between 80 and 90 % ACN. Benzyltrimethylammonium, the smallest quaternary ammonium ion, was the most strongly retained probe at 90 % ACN and was most retained on a high surface area 60 Kromasil column and least retained on a 300 ACE silica gel column. The ionic strength of the mobile phase was varied at 80 and 90 % ACN and plots of log k against the inverse of buffer strength followed by fitting of second-order polynomial curves allowed an assessment of the contribution from HILIC to the mixed HILIC/ion-exchange retention mechanism. Toluene and pentylbenzene were used to assess the decrease in accessible pore volume due to water absorption in HILIC mode.