Abstract
The aim of this research was to identify a biopolymer producing potential actinobacteria candidate from polluted soil. The Streptomyces toxytricini D2 was enumerated as potential biopolymer producer, which was isolated and characterized using 16S rRNA sequencing. The S. toxytricini D2 produced polymeric granules were categorized through FTIR, H-1 NMR, and C-13 NMR analyses, which confirmed that the granules were Polyhydroxyalkanoates (PHAs). The S. toxytricini D2 has the competence to produce 86.56% of PHAs (23.64 g L-1 of PHAs from 27.31 g L-1 of biomass) under optimized growth conditions: 8% of tapioca molasses, 4% of (NH4)(2)SO4, 8% of inoculum, pH 6.5, 30 degrees C and 72 h of incubation. These findings concluded that S. toxytricini D2 could be used as an efficient candidate for the mass production of PHAs polymer under optimized conditions, with a low-cost carbon source and in a short time of production period.