Abstract
A simple and novel capillary zone electrophoresis (CZE) method for simultaneous separation and determination of trovafloxacin mesylate (TRX) and azithromycin (AZ) was developed. The electrophoretic separation was carried out using fused silica capillary (57 cm total length and 50 cm effective length, 75 mu m i.d.) along with an applied electric field of 25 kV and a running buffer containing 25 mmol L-1 sodium tetraborate decahydrate at pH 9.2 and methanol (80:20%) with diode array detection (DAD) at 260 nm. The sample was hydrodynamically anodic injected for 8 s at 90 mbar and the temperature of the capillary cartridge was kept at 30 degrees C. Levofloxacin (LVX) was used as internal standard (IS). A Linear concentration response was recorded over the concentration range of 20-140 mu g mL(-1) with limit of detection 12.0 mu g mL(-1) and limit of quantification 20.0 mu g mL(-1) for both drugs, the correlation coefficient was found to be more than 0.999. Precision study showed that the % RSD was within the range of acceptable limits. The electrophoretic method was successfully validated with respect to sensitivity, linearity, accuracy, precision, ruggedness and robustness. Stability indicating studies for the investigated drugs was examined using the proposed method.