Abstract
A sensitive voltammetric method for detection of trace heavy metal ions using a glassy carbon electrode surfaces chemically modified by a mixture of (SWCNTs/Biomass) was applied. The fungus is used as an inactive biomass, which was selected from soil contaminated with metals. The cell walls have a large number of chemical groups such as carboxyl, sulfonate, amine, hydroxyl, carbonyl and imidazole with which may affect and intervene in the fixing of metal ions. In this assay, heavy metals ions accumulated on the GCE/SWCNTs/Biomass electrode surfaces prior to being subjected to differential pulse anodic stripping voltammetry analysis (DPASV). The resulting peak currents were linearly related to the concentrations of the metal ions. The method was optimized with respect to accumulation time, reduction time and reduction potential. The detection limits were found to be 10−8 M and 10−7 M for Pb2+ and Cd2+ respectively.
•Heavy metal biosensor based on a fungal soil biomass support matrix was developed.•biomass from filamentous fungi was prepared as a homogeneous powder for long-term conservation.•Differential pulse anodic stripping voltammetry was applied to characterize the biomass support matrix.•The developed ion biosensor was used for heavy metal determination.