Abstract
This study was aimed to determine the effect of erythropoietin and insulin on total DNA, Genomic DNA, RNA contents and protein in rat kidney after administration for a short period of seven days. Two doses of erythropoietin (recombinant human erythropoietin EPO) were administered at two different doses of 100 IU/kg and 250 IU/kg and insulin (Rec Hu insulin) at doses of 0.05IU/kg & 0.125 U/kg were daily injected subcutaneously in rats for one week. The kidneys were isolated for extraction of protein and nucleic acids. The genomic DNA was loaded on 1% agarose and RNA was loaded on agarose 1.3%. Proteins were loaded by SDS / PAGE. The results indicated that genomic DNA from rat kidney was 10 kbp after one-week injection of erythropoietin, which was not different from that obtained with control. The molecular size of genomic DNA from rat kidney after one-week injection was similar in both insulin and control groups, which was more than 10 kbp. Protein patterns on SDS/PAGE revealed the same proteins with the same molecular weight between 14 - 66 kDa in all treated and control groups. Similarly, RNA showed no difference in the molecular size (2.5 kbp) between all treated samples after a short-term period.