Abstract
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•Fabrication of a dual-mode electrochemical aptasensor for the detection of Mucin-1 based on AuNPs-magnetic graphene composite.•The fabricated aptasensor exhibited a wide linear range of 10 fM to 10 nM through square wave voltammetry.•A wide linear range of 100fM to 100 nM was observed by chronoamperometry.•The detection limits of the MB-ECAS and H2O2-ECAS are 9.4 fM and 96 fM (S/N = 3), respectively.•The designed aptasensor possess potential application for clinical MUC 1 detection.
Herein, a dual-mode electrochemical aptasensor was proposed for the detection of Mucin-1 (MUC1). AuNPs-Magnetic graphene composite (AuNPs-MG), synthesized through hydrothermal reaction and a subsequent reduction of chloroauric acid in situ, exhibited the ability to absorb methylene blue (MB) by π-π interaction and behavior similar to peroxidase. Moreover, the presence of AuNPs provided the possibility to covalently bond to thiolated DNA. Two electrochemical labels based on the AuNPs-MG were prepared based on this characteristic. In the strand displacement-based electrochemical aptasensor, complementary DNA (CP) in CP-aptamer double-strand DNA exposed owing to the higher affinity between aptamer and MUC1 could capture the electrochemical label through the complementary base pairing principle. Under the optimal conditions, the aptasensor was applied to detect a series concentration of MUC1 by square wave voltammetry (SWV) and chronoamperometry (CA), respectively. The aptasensor shows satisfactory detection results with wide linear ranges of 10 fM to 10 nM through SWV mode and 100fM to 100 nM through CA mode. Meanwhile, the proposed aptasensor with good reproducibility, stability, and possibility in detecting artificial serum samples were obtained, indicating the great potential in clinical analysis applications.