Abstract
A kinetic analysis was made and a linear plot based on the general rate equation derived by Laidler [Can. J. Chem. 33, 1614–1624] is proposed. This linearization method allows determining the kinetic parameters (
K
m,
k
cat) and [E]
0 for enzymes with low catalytic activity. The method was applied to chloroperoxidase from
Caldariomyces fumago [EC 1.11.1.10], whose kinetic parameters
K
m
app,
k
cat
app, and [E]
0 with monochlorodimedone as substrate, were obtained by using the linearization plot and the
V
max value (calculated by Eadie–Hofstee plot).
This plot could also be useful to the study of abenzyme kinetics provided the concentration of the latter is either higher or equal than
K
m value.