Abstract
A new biomimetic sensor (BS) has been prepared by modifying the carbon paste electrode (CPE) based on [Fe(NTB)Cl-2]Cl center dot EtOH (FeC) {NTB = tris(2-benzimidazolylmethyl)amine} for the electrochemical detection of acrylamide (AA). The development of the reported BS was focused on the creation of an AA with alpha-NH2 group of N-terminal valine of hemoglobin (HG), which reduces the peak current of HG-Fe+3 reduction. The fabricated BS was characterized by scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS), and cyclic voltammetry (CV), as well as square wave voltammetry (SWV) for studying the electrochemical properties of AA on its surface. Under optimized conditions, the BS was applied for the direct determination of AA in two linear concentration ranges: 1.0 x 10(-6) and 1.0 x 10(-4) M. The first linear was observed from 1.0 x 10(-6) to 1 x 10(-5) M with a limit of detection (LOD) of 1.465 x 10(-7) M, while the second linear part was detected from 1.0 x 10(-5) to 1 x 10(-4) M with LOD of 5.67 x 10(-6) M. Finally, the constructed BS was successfully applied for the evaluation of AA in various potato chips, and the results were comparable to those obtained from other electrochemical methods. The density functional theory (DFT) could successfully explain the recognition process of acrylamide, that is, the hydroamination of the alkene group of acrylamide by imidazole moiety of the iron complex.