Abstract
The polymorphism at codon 72 of the
TP53 gene has been extensively studied for its involvement in cancerogenesis and loss of heterozygosity (LOH) detection. Usually, the exon 4 of the
TP53 gene is amplified by polymerase chain reaction (PCR) on DNA extracted from blood and tumor tissues, then digested by
AccII. In the case of heterozygosity, the comparison of
AccII profile from blood and tumor DNA PCR products allowed the identification of a potential LOH in the
TP53 locus. This method can be hindered by a partial
AccII digestion and/or DNA contamination of non-tumor cells. To circumvent these problems, we have developed a new approach by using the AccII restriction site between exon 4 and exon 6. The PCR amplification of exon 4–6, followed by
AccII digestion allowed us to detect without ambiguity any LOH case.