Abstract
Acute skin wounds often lead to tissue defects and bacterial infection, leading to the suffering of patients. In this study, we reported a multifunctional nanofiber reinforced photo-crosslinking hydrogel wound dressing mainly based on natural biomaterials to mimic the structure and components of the native extracellular matrix (ECM). Gelatin-methacryloyl (GelMA), Thioglycolic acid-modified chitosan (TCS) and 3-buten-1-amine (BA) modified polycaprolactone nanofiber (PCLPBA) were prepared and photo-crosslinked into GTP hydrogel. Among all hydrogels, the GTP4 hydrogel possessed rapid liquid-gel transition at ultraviolet (UV) exposure, excellent anti-bacterial effect, antioxidant property, and printability through 3D printing. Besides, NIH 3T3 cells proliferated well and invaded GTP4 hydrogel. When it was applied to the full-thickness skin defect, GTP4 hydrogel showed the enhanced angiogenesis, re-epithelialization and wound healing effect. Therefore, these findings indicate that the GTP4 hydrogel is a promising wound dressing for acute skin wounds.