A multilaboratory comparison of calibration accuracy and the performance of external references in analytical ultracentrifugation

Huaying Zhao; Rodolfo Ghirlando; Carlos Alfonso; Fumio Arisaka; Ilan Attali; David L Bain; Marina M Bakhtina; Donald F Becker; Gregory J Bedwell; Ahmet Bekdemir; Tabot M D Besong; Catherine Birck; Chad A Brautigam; William Brennerman; Olwyn Byron; Agnieszka Bzowska; Jonathan B Chaires; Catherine T Chaton; Helmut Cölfen; Keith D Connaghan; Kimberly A Crowley; Ute Curth; Tina Daviter; William L Dean; Ana I Díez; Christine Ebel; Debra M Eckert; Leslie E Eisele; Edward Eisenstein; Patrick England; Carlos Escalante; Jeffrey A Fagan; Robert Fairman; Ron M Finn; Wolfgang Fischle; José García de la Torre; Jayesh Gor; Henning Gustafsson; Damien Hall; Stephen E Harding; José G Hernández Cifre; Andrew B Herr; Elizabeth E Howell; Richard S Isaac; Shu-Chuan Jao; Davis Jose; Soon-Jong Kim; Bashkim Kokona; Jack A Kornblatt; Dalibor Kosek; Elena Krayukhina; Daniel Krzizike; Eric A Kusznir; Hyewon Kwon; Adam Larson; Thomas M Laue; Aline Le Roy; Andrew P Leech; Hauke Lilie; Karolin Luger; Juan R Luque-Ortega; Jia Ma; Carrie A May; Ernest L Maynard; Anna Modrak-Wojcik; Yee-Foong Mok; Norbert Mücke; Luitgard Nagel-Steger; Geeta J Narlikar; Masanori Noda; Amanda Nourse; Tomas Obsil; Chad K Park; Jin-Ku Park; Peter D Pawelek; Erby E Perdue; Stephen J Perkins; Matthew A Perugini; Craig L Peterson; Martin G Peverelli; Grzegorz Piszczek; Gali Prag; Peter E Prevelige; Bertrand D E Raynal; Lenka Rezabkova; Klaus Richter; Alison E Ringel; Rose Rosenberg; Arthur J Rowe; Arne C Rufer; David J Scott; Javier G Seravalli; Alexandra S Solovyova; Renjie Song; David Staunton; Caitlin Stoddard; Katherine Stott; Holger M Strauss; Werner W Streicher; John P Sumida; Sarah G Swygert; Roman H Szczepanowski; Ingrid Tessmer; Ronald T Toth, 4th; Ashutosh Tripathy; Susumu Uchiyama; Stephan F W Uebel; Satoru Unzai; Anna Vitlin Gruber; Peter H von Hippel; Christine Wandrey; Szu-Huan Wang; Steven E Weitzel; Beata Wielgus-Kutrowska; Cynthia Wolberger; Martin Wolff; Edward Wright; Yu-Sung Wu; Jacinta M Wubben; Peter Schuck

doi:10.1371/journal.pone.0126420

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A multilaboratory comparison of calibration accuracy and the performance of external references in analytical ultracentrifugation

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A multilaboratory comparison of calibration accuracy and the performance of external references in analytical ultracentrifugation

Huaying Zhao, Rodolfo Ghirlando, Carlos Alfonso, Fumio Arisaka, Ilan Attali, David L Bain, Marina M Bakhtina, Donald F Becker, Gregory J Bedwell, Ahmet Bekdemir, …

PloS one, Vol.10(5), pp.e0126420-e0126420

21/05/2015

DOI: https://doi.org/10.1371/journal.pone.0126420

PMCID: PMC4440767

PMID: 25997164

Abstract

Calibration

Reproducibility of Results

Ultracentrifugation - methods

Ultracentrifugation - standards

Analytical ultracentrifugation (AUC) is a first principles based method to determine absolute sedimentation coefficients and buoyant molar masses of macromolecules and their complexes, reporting on their size and shape in free solution. The purpose of this multi-laboratory study was to establish the precision and accuracy of basic data dimensions in AUC and validate previously proposed calibration techniques. Three kits of AUC cell assemblies containing radial and temperature calibration tools and a bovine serum albumin (BSA) reference sample were shared among 67 laboratories, generating 129 comprehensive data sets. These allowed for an assessment of many parameters of instrument performance, including accuracy of the reported scan time after the start of centrifugation, the accuracy of the temperature calibration, and the accuracy of the radial magnification. The range of sedimentation coefficients obtained for BSA monomer in different instruments and using different optical systems was from 3.655 S to 4.949 S, with a mean and standard deviation of (4.304 ± 0.188) S (4.4%). After the combined application of correction factors derived from the external calibration references for elapsed time, scan velocity, temperature, and radial magnification, the range of s-values was reduced 7-fold with a mean of 4.325 S and a 6-fold reduced standard deviation of ± 0.030 S (0.7%). In addition, the large data set provided an opportunity to determine the instrument-to-instrument variation of the absolute radial positions reported in the scan files, the precision of photometric or refractometric signal magnitudes, and the precision of the calculated apparent molar mass of BSA monomer and the fraction of BSA dimers. These results highlight the necessity and effectiveness of independent calibration of basic AUC data dimensions for reliable quantitative studies.

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Title: A multilaboratory comparison of calibration accuracy and the performance of external references in analytical ultracentrifugation
Creators - without role: Huaying Zhao - National Institute of Biomedical Imaging and Bioengineering
Rodolfo Ghirlando - National Institute of Diabetes and Digestive and Kidney Diseases
Carlos Alfonso - Centro de Investigaciones Biológicas Margarita Salas
Fumio Arisaka - Nihon University
Ilan Attali - Tel Aviv University
David L Bain - University of Colorado Anschutz Medical Campus
Marina M Bakhtina - The Ohio State University
Donald F Becker - University of Nebraska–Lincoln
Gregory J Bedwell - University of Alabama at Birmingham
Ahmet Bekdemir - École Polytechnique Fédérale de Lausanne
Tabot M D Besong - University of Nottingham
Catherine Birck - Institute of Genetics and Molecular and Cellular Biology
Chad A Brautigam - The University of Texas Southwestern Medical Center
William Brennerman - Beckman Coulter Foundation
Olwyn Byron - University of Glasgow
Agnieszka Bzowska - University of Warsaw
Jonathan B Chaires - University of Louisville
Catherine T Chaton - University of Cincinnati Medical Center
Helmut Cölfen - University of Konstanz
Keith D Connaghan - University of Colorado Anschutz Medical Campus
Kimberly A Crowley - University of Massachusetts Chan Medical School
Ute Curth - Medizinische Hochschule Hannover
Tina Daviter - Birkbeck, University of London
William L Dean - University of Louisville
Ana I Díez - Universidad de Murcia
Christine Ebel - Université Grenoble Alpes
Debra M Eckert - University of Utah
Leslie E Eisele - New York State Department of Health
Edward Eisenstein - Advanced Bioscience Laboratories
Patrick England - Institut Pasteur
Carlos Escalante - Virginia Commonwealth University
Jeffrey A Fagan - National Institute of Standards and Technology
Robert Fairman - Haverford College
Ron M Finn - Max Planck Institute for Biophysical Chemistry
Wolfgang Fischle - Max Planck Institute for Biophysical Chemistry
José García de la Torre - Universidad de Murcia
Jayesh Gor - Institute of Structural and Molecular Biology
Henning Gustafsson - Novo Nordisk
Damien Hall - Australian National University
Stephen E Harding - University of Nottingham
José G Hernández Cifre - Universidad de Murcia
Andrew B Herr - University of Cincinnati Medical Center
Elizabeth E Howell - University of Tennessee at Knoxville
Richard S Isaac - University of California, San Francisco
Shu-Chuan Jao - Institute of Biological Chemistry, Academia Sinica
Davis Jose - University of Oregon
Soon-Jong Kim - Mokpo National University
Bashkim Kokona - Haverford College
Jack A Kornblatt - Concordia University
Dalibor Kosek - Charles University
Elena Krayukhina - Osaka University
Daniel Krzizike - Colorado State University
Eric A Kusznir - Roche
Hyewon Kwon - University of Washington
Adam Larson - University of California, San Francisco
Thomas M Laue - University of New Hampshire
Aline Le Roy - Université Grenoble Alpes
Andrew P Leech - University of York
Hauke Lilie - Martin Luther University Halle-Wittenberg
Karolin Luger - Colorado State University
Juan R Luque-Ortega - Centro de Investigaciones Biológicas Margarita Salas
Jia Ma - National Institute of Biomedical Imaging and Bioengineering
Carrie A May - University of New Hampshire
Ernest L Maynard - Uniformed Services University of the Health Sciences
Anna Modrak-Wojcik - University of Warsaw
Yee-Foong Mok - University of Melbourne
Norbert Mücke - German Cancer Research Center
Luitgard Nagel-Steger - Forschungszentrum Jülich
Geeta J Narlikar - University of California, San Francisco
Masanori Noda - Osaka University
Amanda Nourse - St. Jude Children's Research Hospital
Tomas Obsil - Charles University
Chad K Park - University of Arizona
Jin-Ku Park - Mokpo National University
Peter D Pawelek - Concordia University
Erby E Perdue - Beckman Coulter Foundation
Stephen J Perkins - University College London
Matthew A Perugini - La Trobe University
Craig L Peterson - University of Massachusetts Chan Medical School
Martin G Peverelli - La Trobe University
Grzegorz Piszczek - National Heart Lung and Blood Institute
Gali Prag - Tel Aviv University
Peter E Prevelige - University of Alabama at Birmingham
Bertrand D E Raynal - Institut Pasteur
Lenka Rezabkova - Paul Scherrer Institute
Klaus Richter - Center for Integrated Protein Science Munich
Alison E Ringel - Johns Hopkins Medicine
Rose Rosenberg - University of Konstanz
Arthur J Rowe - University of Nottingham
Arne C Rufer - Roche
David J Scott - Research Complex at Harwell
Javier G Seravalli - University of Nebraska–Lincoln
Alexandra S Solovyova - Newcastle University
Renjie Song - New York State Department of Health
David Staunton - Molecular Biophysics Suite, Department of Biochemistry, Oxford, Oxon, OX1 3QU, United Kingdom
Caitlin Stoddard - University of California, San Francisco
Katherine Stott - University of Cambridge
Holger M Strauss - Novo Nordisk
Werner W Streicher - Novo Nordisk Foundation
John P Sumida - University of Washington
Sarah G Swygert - University of Massachusetts Chan Medical School
Roman H Szczepanowski - International Institute of Molecular and Cell Biology
Ingrid Tessmer - University of Würzburg
Ronald T Toth, 4th - University of Kansas
Ashutosh Tripathy - University of North Carolina at Chapel Hill
Susumu Uchiyama - Osaka University
Stephan F W Uebel - Max Planck Institute of Biochemistry
Satoru Unzai - Yokohama City University
Anna Vitlin Gruber - Tel Aviv University
Peter H von Hippel - Institute of Molecular Biology and Department of Chemistry and Biochemistry, University of Oregon, Eugene, Oregon, 97403, United States of America
Christine Wandrey - École Polytechnique Fédérale de Lausanne
Szu-Huan Wang - Academia Sinica
Steven E Weitzel - Institute of Molecular Biology and Department of Chemistry and Biochemistry, University of Oregon, Eugene, Oregon, 97403, United States of America
Beata Wielgus-Kutrowska - University of Warsaw
Cynthia Wolberger - Johns Hopkins University School of Medicine
Martin Wolff - ICS-6, Structural Biochemistry, Research Center Juelich, 52428, Juelich, Germany
Edward Wright - University of Tennessee at Knoxville
Yu-Sung Wu - University of Delaware
Jacinta M Wubben - La Trobe University
Peter Schuck - National Institute of Biomedical Imaging and Bioengineering
Publication Details: PloS one, Vol.10(5), pp.e0126420-e0126420
Grant note: GM-15792 / NIGMS NIH HHS GM 094363 / NIGMS NIH HHS F31 GM105363 / NIGMS NIH HHS R01 GM067777 / NIGMS NIH HHS R01 GM095822 / NIGMS NIH HHS GM 095822 / NIGMS NIH HHS GM067777 / NIGMS NIH HHS P30 GM103519 / NIGMS NIH HHS R01 GM029158 / NIGMS NIH HHS F31GM105363 / NIGMS NIH HHS P50 GM082545 / NIGMS NIH HHS R01 GM109102 / NIGMS NIH HHS CA35635 / NCI NIH HHS P30GM103519 / NIGMS NIH HHS R01 CA035635 / NCI NIH HHS R01 GM015792 / NIGMS NIH HHS R01 GM054096 / NIGMS NIH HHS GM-29158 / NIGMS NIH HHS P30 GM103335 / NIGMS NIH HHS R01 GM094363 / NIGMS NIH HHS GM82545 / NIGMS NIH HHS GM 109102 / NIGMS NIH HHS
Identifiers: 9942573308331
Academic Unit: King Abdullah University of Science & Technology
Language: English
Resource Type: Journal article