Abstract
A new sterol, ergosta-8(9),22-diene-3,5,6,7-tetraol(3beta,5alpha,6beta,7alpha,22E)(A) together with three known sterols: 30, 5alpha,6beta-trihydroxyergosta-7,22-diene (B), 3beta-hydroxy-5alpha,8alpha- epidioxyergosta-6,22-diene (C) and ergosterol (D) were isolated from the mycelia of an unidentified endophytic fungus separated from Castaniopsis fissa (chestnut tree). Compound A exhibited potent selective cytotoxicity against Bel-7402, NC14460 and L-02 cell lines with IC50 values 8.445, 5.03, 13.621 mug/mL, respectively.