A new thio-Schiff base fluorophore with copper ion sensing, DNA binding and nuclease activity

R. Vikneswaran; Muhamad Syamir Syafiq; Naser Eltaher Eltayeb; Mohd Naqiuddin Kamaruddin; S. Ramesh; R. Yahya

doi:10.1016/j.saa.2015.05.087

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A new thio-Schiff base fluorophore with copper ion sensing, DNA binding and nuclease activity

Journal article

Peer reviewed

A new thio-Schiff base fluorophore with copper ion sensing, DNA binding and nuclease activity

R. Vikneswaran, Muhamad Syamir Syafiq, Naser Eltaher Eltayeb, Mohd Naqiuddin Kamaruddin, S. Ramesh and R. Yahya

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, Vol.150, pp.175-180

05/11/2015

DOI: https://doi.org/10.1016/j.saa.2015.05.087

PMID: 26046495

Abstract

Chemical nucleases

Chemosensor

DNA binding

Fluorescent

Thiosemicarbazone

[Display omitted] •A white light-emitting Schiff base.•An excellent fluorescence probe for Cu2+ detection.•Binds DNA electrostatically.•Mimics nuclease behavior. Copper ion recognition and DNA interaction of a newly synthesized fluorescent Schiff base (HPyETSC) were investigated using UV–vis and fluorescent spectroscopy. Examination using these two techniques revealed that the detection of copper by HPyETSC is highly sensitive and selective, with a detection limit of 0.39μm and the mode of interaction between HPyETSC and DNA is electrostatic, with a binding constant of 8.97×104M−1. Furthermore, gel electrophoresis studies showed that HPyETSC exhibited nuclease activity through oxidative pathway.

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Title: A new thio-Schiff base fluorophore with copper ion sensing, DNA binding and nuclease activity
Creators - without role: R. Vikneswaran - University of Malaya
Muhamad Syamir Syafiq - University of Malaya
Naser Eltaher Eltayeb - King Abdulaziz University
Mohd Naqiuddin Kamaruddin - Centre for Ionics University of Malaya, Department of Physics, Faculty of Science, University of Malaya, Lembah Pantai, 50603 Kuala Lumpur, Malaysia
S. Ramesh - Centre for Ionics University of Malaya, Department of Physics, Faculty of Science, University of Malaya, Lembah Pantai, 50603 Kuala Lumpur, Malaysia
R. Yahya - University of Malaya
Publication Details: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, Vol.150, pp.175-180
Publisher: Elsevier B.V
Identifiers: 9934801508331
Academic Unit: King Abdulaziz University
Language: English
Resource Type: Journal article