Abstract
The phytochemical study of the aerial parts of Bupleurum marginatum revealed a novel aryltetraline lactone lignan (9) along with 14 known compounds. Their structures have been determined by 1D- and 2D-NMR and MS analyses. The isolated lignans possessed cytotoxic activity against HepG2 and HeLa cells. [Display omitted]
► A detailed phytochemical study of the aerial parts extract led to isolation and characterization of 15 compounds. ► A new aryltetraline lactone lignan was identified as marginatoxin. ► The cytotoxic activity of the isolated compounds in both HepG2 and HeLa cancer cell lines are reported.
A detailed phytochemical study of the aerial parts of Bupleurum marginatum Wall. ex DC revealed a novel aryltetraline lactone lignan identified as 9-(benzo[d][1,3]dioxol-5-yl)-6,7,8-trimethoxy-3a,4,9,9a-tetrahydronaphtho[2,3-c]furan-1(3H)-one (marginatoxin) along with nine known compounds and characterization of five other compounds either by GLC/MS or LC/MS techniques. Chemical structures of the isolated compounds were unambiguously elucidated by both 1D, 2D NMR and mass spectrometry techniques.
The in vitro cytotoxic activity of both methanol and dichloromethane extracts as well as the isolated compounds was assessed in two human cancer cell lines HepG2 and HeLa using the MTT assay. The new aryltetraline lactone lignan exhibited a potent cytotoxic activity with IC50 values of 12.14 and 16.90μM after 24h treatment for HepG2 and HeLa cells, respectively.