Abstract
Based on Ru(bpy)(3)(2+)-Au nanoparticles decorated multi-walled carbon nanotubes composites and a molecularly imprinted polymer (MIP), we propose a novel molecularly imprinted electrochemiluminescence (ECL) sensor to selectively determine isoniazid (INH). The MIP is synthesized through electrochemical copolymerization of acrylamide and N,N'-methylene diacrylamide in the presence of INH template molecules. The enhanced ECL intensity is linear in the range of 0.1 to 110 mu g cm(-3) and the detection limit is 0.08 mu g cm(-3) (3 sigma) INH with relative standard deviation 3.8% (n = 6) for 8 mu g cm(-3). As a result, the sensor has been successfully applied to the determination of INH in human urine and pharmaceutical samples. Moreover, the possible ECL mechanism is discussed.