Abstract
In the exploration for novel drugs, medicinal plants used traditional medicines are promising candidates. For the naturally rare, seasonally dependent, and slowly growing plant species, plant tissue culture offers a cost-effective, plant available all year round, and well-controlled means for mass production of the active values of medicinal plants for pharmaceutical industries. This study was aimed to generate callus from Rosa damascena flowering buds in vitro for the first time, and evaluate whether the generated callus has therapeutic effect as anti-inflammatory agent to suppress the activated T-cells from human blood.
The flowering buds of Rosa. damascina were prepared and cultured in full strength Murashige and Skoog medium supplemented with kinetin and naphthaleneacetic acid. The callus were collected freshly after 40 days and, dried until dryness. Powdered callus was extracted with methanol by Soxhlet apparatus for about 6 hrs at 60 degrees C. The generated extract was used to treat Human PBMCs after activating T-cells with anti-human CD3 and CD28. The results from this study showed development of a new method to culture R. damascena flowering buds in vitro. The callus obtained is capable of suppressing the activated T-cells and thus can reduce the inflammation.