Abstract
A reliable sensor for the detection of testosterone was developed by the deposition CuO/CeO
2
nanocomposites (NCs) onto glassy carbon electrode (GCE). The wet chemically prepared NCs were totally characterized in detail by X-ray photoelectron spectroscopy, ultraviolet–visible spectroscopy, field emission scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction analysis, and energy-dispersive X-ray spectroscopy analysis. In this electrochemical (
I
–
V
) approach, a current versus concentration of testosterone is plotted, which is known as calibration of testosterone sensor. It is obtained the linearly over the concentration range of 0.1 to 0.01 nM, which is denoted as a linear dynamic range (LDR). The sensor sensitivity is achieved from the slope of the calibration curve by considering the active surface area of GCE (0.0316 cm
2
), which is equal to 27.3576 µA µM
−1
cm
−2
. The lower limit for the detection of testosterone is calculated (as 9.30 ± 0.47 pM) from signal–noise ratio at 3. Besides this, the response time, long-time performing stability, and reproducibility of sensor are found reliable and good. The biological samples such as human, mouse, and rabbit serums were tested for the validity of the proposed sensor probe to the selective detection.