Abstract
We use a rate equation model for the loss of the photosensitizer resulting from photo-oxidation (singlet oxygen attack on the photodynamic therapy (PDT) photosensitizer). It also incorporates the effect of photobleaching (photosensitizer decay) on the oxygen and the photosensitizer concentration which play an important role in photodynamic damage in different cell cultures. As a part of this study, we calculated photodynamic damage against the exposure time. Secondly photodynamic damage in different treated malignant/neoplastic as well as normal cell lines (Hep-2, A-549, and WI-38) has been investigated using a continuous wave (CW) He-Ne laser (633 nm of red wavelength). For determination of effectiveness of photodynamic damage two sorts of experiments are conducted. In first step of this experiment, given cell lines were photosensitized/treated with 1 ml of delta-aminolevulinic acid (ALA) having working solution of 200 mu g/ml and after 4 hours of time of incubation ALA treated cells were exposed/irradiated with different doses (15-60 J/cm(2)) with constant irradiation time (15 minutes approximately). Same technique with exception of different concentration of ALA (60-800 mu g/ml) and light dose (15-60 J/cm(2) /5-20 mW of power along with time of irradiation 15 minutes approximately) has been applied in second step of experiment. Finally result has been verified by using staining of mitochondria technique. 633 nm He-Ne (CW) along with 400 mu g/ml of 5-ALA stipulates excellent therapeutic results verified by using mitochondrial staining technique as well as cell hemocytometry. Both the experimental as well as theoretical results are in good agreement with each other. (C) 2012 by Astro, Ltd.