Abstract
This study's aim was to investigate iron (Fe) and zinc (Zn) concentration in the soil, forage crops, and sheep blood with respect to the seasonal availability of these metals. Soil, forage, and sheep blood samples were sampled from five different locations in Chakwal (Pidh, Tobar, Ratoccha, Choa Saiden Shah-Kalar Kahar road, and Choa Saiden Shan-Chakwal Road) during two seasons, i.e., winter and summer. All the samples were processed through wet acid digestion for evaluation of metal contents. Because of proximity of site-1 and site-2 to coal mines, higher Fe concentration was observed than Zn. Overall, varied Fe concentrations obtained in soil were 12.95-24.31 mg/kg, 1.29-9.61 mg/kg in forage and 1.17-24 mg/l in blood, whereas Zn values were 1.04-31.9 mg/kg, 1.96-7.02 mg/kg, and 0.16-6.52 mg/l for soil, forages, and blood respectively. The pollution load index value for both Fe (0.01-0.14 mg/kg) and Zn (0.02-0.72 mg/kg) was lesser than 1. Bio-concentration (0.09-2.64mg/kg) and enrichment factor (0.08-7.51 mg/kg) were showing efficient transfer of metals through the food chain. Daily intake and health risk index values of iron were ranged from 0.01 to 1.1 mg/kg/day and 0.02 to 1.05 mg/kg/day. There was a probable chance of upsurge in metal values in coming years due to continued mining activities. Anthropogenic input, mainly mining activities in the study area, have increased the Fe and Zn content in the environment which can ultimately find their way up the food chain, thereby risking the health of grazing livestock.