Abstract
The aim of this study was to investigate the antioxidant activity and inhibitory effect of 2,4,4'-trihydroxychalcone on digestive enzymes related to obesity, including sucrase, alpha-amylase, and lipase. The in vitro antioxidant activities of three concentrations of 2,4,4'-trihydroxychalcone (100, 300, and 500 mu g/ml) were determined using 2,2-diphenyl-1-picrylhydrazyl radical scavenging, reducing power, and ferrous ion chelating assays. Moreover, in vitro inhibition of lipase, alpha-amylase, and sucrase enzyme activities by 2,4,4'-trihydroxychalcone was determined using a specific assay for each enzyme. 2,4,4'-Trihydroxychalcone has been shown to have antioxidant properties and inhibits sucrase, alpha-amylase and lipase activities. These findings suggest that 2,4,4'-trihydroxychalcone demonstrated an antioxidant activity and can effectively inhibit the key enzymes related to obesity.