Abstract
Exposure of water to a magnetic field increases its solubility, conductivity, and mineral content. Previous studies have shown that magnetized water can cause weight gain, increase milk production. and help treat diseases in livestock. This study aimed to evaluate magnetized water's antioxidant activity in oxidatively stressed mice by Nerium oleander ethanolic extract.
Forty 6- to 8-week-old Blab/c mice were classified into four groups: control, magnetized water. Nerium oleander, and magnetized water plus Nerium oleander. The weight of each mouse was measured at the beginning of the experiment, after 3 and 6 weeks, and at the end of the experiment. After 6 weeks, a blood sample was collected from each mouse in plain and EDTA tubes to measure hematological and chemical parameters, respectively. Moreover, the mice were sacrificed, and then the liver, kidney, and heart were immediately collected and washed with saline and used for measuring oxidants and antioxidant parameters and for histopathological examination. In addition to scrum ALT, AST, total CK, CK-MB, and c-troponin I levels, the following hematological parameters were measured: Red blood cell (RBC) count, hemoglobin, hematocrit, and MCV. Moreover, lipid peroxidation (LPO), carbonyl carbon (CC), reduced glutathione (GSH), catalase, glutathione peroxidase (GSH Px), and superoxide dismutase (SOD) were measured in hepatic, kidney, and cardiac homogenates.
After 6 weeks and at the end of the experiment, the mice in the magnetized water group had a significantly higher weight gain (p < 0.05 and p < 0.01, respectively). The hematological parameters did not show statistically significant differences among the groups. Serum AST, ALT, total CK. CK-MB, and ctroponin I levels showed statistically significant differences between the four groups (p < 0.01). In the cardiac homogenate, both LPO and CC levels were significantly reduced in the magnetized water group, while GSH was increased (p < 0.01). Moreover. catalase. GSH-Px, and SOD activities were significantly elevated in the magnetized water group in the same homogenate. In the hepatic homogenate. the activity of the antioxidant enzymes catalase, GSH-Px, and SOD was significantly increased in the magnetized water group (p < 0.05). Finally, the activity of catalase was significantly increased in the kidney homogenate of the magnetized water group (p < 0.05). The histopathologic appearance of liver and cardiac tissues induced by injection of mice with Nerium oleander were milder in group drinking magnetized water.
The present study concludes that magnetized water increased the activity of the antioxidant enzymes catalase, glutathione peroxidase, and superoxide dismutase. These enzymes increased the activity of antioxidant molecules and reduced glutathione, while they decreased lipid peroxidation and carbonyl carbon during oxidative stress in mice. Moreover, magnetized water caused a weight gain in these mice, resulting from the increased solubility of this water for minerals and vitamins.