Abstract
The purpose of this study was to determine the phytochemical analysis, antioxidant activity and chemical components of ethayl acetate extract of T. aphylla from Saudi cultivar. The objective of this study was to characterize the extract for its phytochemical composition and antioxidant activity. Phytochemical analysis of crude extracts for T. aphylla detected the presence of alkaloids, flavonoids, tannins, and saponins, whereas, both steroids and triterpenoids were absent. The maximum total phenol content was 39.3 mg gallic acid / g dry extract. The antioxidant activity was evaluated by DPPH assay; concentration of T. aphylla 1, 5, 10, 15, 20 and 25 mg/ml corresponed to scavenging activities of similar to 22.1%, 31.4%, 39.2%, 53.5, 60.0 and 66.3%, respectively. While for ascorbic acid (Standard), the scavenging activities were 33.1, 46.2, 51.6, 58.2, 67.3 and 72.2% at the same concentrations. IC50 value of T. aphylla was 14.0 mg/ml whereas it was 9.0 mg/ml for ascorbic acid. Addition GC -MS chromatogram of T. aphylla ethyl acetate extract were presented twenty-three constituents.