Abstract
Background: APOBEC3G (Apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like 3G) gene is one of the genetic host factors, have been linked with HIV-1 AIDS predisposing and protection in different residence populations. The investigation of genetic marker (APOBEC3G) variant (rs6001417) CC, CG and GG genotypes in Pakistan.
Methods: The extraction of DNA, the DNA Rapid Salting-out method was used. Then the observed DNA with electrophoresis technique referred for quantitative real-time PCR to identify the APOBEC3G variant rs6001417 genotypes and Taq Man genotyping.
Results: Three genotypes of rs6001417 (CC, CG and GG) were compared both in HIV-1 infected patients and healthy control groups (p=0.73, p=0.007, p=0.01 respectively). The rs6001417 CG and GG genotype demonstrated a significant involvement in both the healthy and infected individuals and portraying possible protective effect against HIV-1 infection with predictive value of 36.43% and 13.57% respectively.
Conclusion: APOBEC3G (rs6001417) CG and GG genotypes may have a protective feature in the progression of HIV-1 infection and we may use this as a preliminary predictive marker in the country for HIV-1 infected individuals as well.