Abstract
Arcanobacterium (Actinomyces) pyogenes, are animal pathogens, they produce hemolytic exotoxin, pyolysin (PLO) and are opportunistic pathogens of economically important livestock such as dairy, beef cattle and sheep. Samples were collected from pleural fluid of 10 diseased and 10 healthy sheep. Samples were from neighboring village to gazelle breeding center, Riyadh Saud Arabia. The objectives of this study were to investigate A. pyogenes in these sheep. The isolates were grown on brain heart infusion (BHI; Difco) agar plates, supplemented with 5% bovine blood at 37 degrees C and 5% CO2. Biochemical identification of 20 isolates revealed beta-hemolytic on blood agar, hydrolyzed gelatin, starch, were unable to hydrolyze esculin or reduce nitrate and did not produce urease. DNA was extracted from isolates using DNeasy Blood and Tissue Kits (Qiagen, NY, USA). Molecular tool using 16S r-deoxyribonucleic acid (DNA) universal eubacterial forward primer 16F27 (5'-AGA GTT TGA TCC TGG CTC AG-3') and reverse primer 16SrDNA R1525 (5'-AAG GAG GTG ATC CAG CCG CA-3'), MWG Biotech AG (Ebersberg, Germany) identified the isolates successfully as A. pyogenes.