Abstract
A new simple, sensitive and reliable adsorptive and affinity linear sweep voltammetric method at in situ mercury film electrode for the determination of singulair in tablets and its interaction with human serum albumin (HSA) was investigated. The solubility of singulair in aqueous media was enhanced by inclusion with hydroxypropyl-beta-cyclodextrin (HP beta CD). The nature of the electrochemical process of singulair was studied by affinity cyclic voltammetry (ACV). Reproducibility of the proposed method was checked giving interday precision of 0.073 standard deviation. The limit of detection and limit of quantification were 0.05 and 0.16 fg/L, respectively. In the present work, singulair was interacted with HSA by 1: 1 stoichiometry to form electroinactive supramolecular complex. The binding constant was precisely estimated by coupling affinity linear sweep voltammetry (ALSV) and ACV with non-linear regression analysis based on the shifting of analyte peak potentials. The proposed experiments and data analysis could be used to investigate the binding constant of drug with protein within one hour.