Abstract
Background: The exceptional characteristics of cerium oxide (CeO
2
) and aluminum oxide (Al
2
O
3
) nanoscales have inspired significant attention to those nanocomposites as possible electroactive resources for applications of sensing and biosensing. Methods: In this research, an innovative new factionalized CeO
2
/Al
2
O
3
nanocomposite membrane sensor was presented to assess tramadol hydrochloride (TRD) in marketable products. Results: Tramadol-phosphomolybdate (TRD-PM) was formed by mixing tramadol hydrochloride and phosphomolybdic acid (PMA) in the attendance of polymeric matrix and
o
-nitrophenyloctyl ether solvent mediator. With 1.0 × 10
−10
–1.0 × 10
−2
mol L
−1
as a range of linearity and E
mV
= (57.567 ± 0.2) log [TRD] + 676.29 as a regression equation, the functionalized sensor using TRD-PM-CeO
2
/Al
2
O
3
nanocomposite showed great selectivity and sensitivity for the discriminating and measurement of TRD. Using the regression equation E
mV
= (52.143 ± 0.4) log [TRD] + 431.45, the unmodified coated wire sensor of TRD-PM, on the other hand, showed a Nernstian response between 1.0 × 10
−6
and 1.0 × 10
−2
mol L
−1
, Using the methodology’s specified guidelines, the proposed improved potentiometric system was validated against several criteria. Conclusion: The suggested method is suitable for the determination of TRD in its products.