Aegilops sharonensis genome-assisted identification of stem rust resistance gene Sr62

Guotai Yu; Oadi Matny; Nicolas Champouret; Burkhard Steuernagel; Matthew J Moscou; Inmaculada Hernández-Pinzón; Phon Green; Sadiye Hayta; Mark Smedley; Wendy Harwood; Ngonidzashe Kangara; Yajuan Yue; Catherine Gardener; Mark J Banfield; Pablo D Olivera; Cole Welchin; Jamie Simmons; Eitan Millet; Anna Minz-Dub; Moshe Ronen; Raz Avni; Amir Sharon; Mehran Patpour; Annemarie F Justesen; Murukarthick Jayakodi; Axel Himmelbach; Nils Stein; Shuangye Wu; Jesse Poland; Jennifer Ens; Curtis Pozniak; Miroslava Karafiátová; István Molnár; Jaroslav Doležel; Eric R Ward; T Lynne Reuber; Jonathan D G Jones; Martin Mascher; Brian J Steffenson; Brande B H Wulff

doi:10.1038/s41467-022-29132-8

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Aegilops sharonensis genome-assisted identification of stem rust resistance gene Sr62

Journal article

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Aegilops sharonensis genome-assisted identification of stem rust resistance gene Sr62

Guotai Yu, Oadi Matny, Nicolas Champouret, Burkhard Steuernagel, Matthew J Moscou, Inmaculada Hernández-Pinzón, Phon Green, Sadiye Hayta, Mark Smedley, Wendy Harwood, …

Nature communications, Vol.13(1), pp.1607-1607

25/03/2022

DOI: https://doi.org/10.1038/s41467-022-29132-8

PMID: 35338132

Abstract

Aegilops - genetics

Basidiomycota - genetics

Disease Resistance - genetics

Genes, Plant - genetics

Plant Breeding

Plant Diseases - genetics

Triticum - genetics

The wild relatives and progenitors of wheat have been widely used as sources of disease resistance (R) genes. Molecular identification and characterization of these R genes facilitates their manipulation and tracking in breeding programmes. Here, we develop a reference-quality genome assembly of the wild diploid wheat relative Aegilops sharonensis and use positional mapping, mutagenesis, RNA-Seq and transgenesis to identify the stem rust resistance gene Sr62, which has also been transferred to common wheat. This gene encodes a tandem kinase, homologues of which exist across multiple taxa in the plant kingdom. Stable Sr62 transgenic wheat lines show high levels of resistance against diverse isolates of the stem rust pathogen, highlighting the utility of Sr62 for deployment as part of a polygenic stack to maximize the durability of stem rust resistance.

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Title: Aegilops sharonensis genome-assisted identification of stem rust resistance gene Sr62
Creators - without role: Guotai Yu - Norwich Research Park
Oadi Matny - University of Minnesota
Nicolas Champouret - Syngenta
Burkhard Steuernagel - Norwich Research Park
Matthew J Moscou - Sainsbury Laboratory
Inmaculada Hernández-Pinzón - Sainsbury Laboratory
Phon Green - Sainsbury Laboratory
Sadiye Hayta - Norwich Research Park
Mark Smedley - Norwich Research Park
Wendy Harwood - Norwich Research Park
Ngonidzashe Kangara - Norwich Research Park
Yajuan Yue - Norwich Research Park
Catherine Gardener - Norwich Research Park
Mark J Banfield - Norwich Research Park
Pablo D Olivera - University of Minnesota
Cole Welchin - University of Minnesota
Jamie Simmons - University of Minnesota
Eitan Millet - Tel Aviv University
Anna Minz-Dub - Tel Aviv University
Moshe Ronen - Tel Aviv University
Raz Avni - Tel Aviv University
Amir Sharon - Tel Aviv University
Mehran Patpour - Aarhus University
Annemarie F Justesen - Aarhus University
Murukarthick Jayakodi - Leibniz Institute of Plant Genetics and Crop Plant Research
Axel Himmelbach - Leibniz Institute of Plant Genetics and Crop Plant Research
Nils Stein - Leibniz Institute of Plant Genetics and Crop Plant Research
Shuangye Wu - Kansas State University
Jesse Poland - Kansas State University
Jennifer Ens - University of Saskatchewan
Curtis Pozniak - University of Saskatchewan
Miroslava Karafiátová - Czech Academy of Sciences, Institute of Experimental Botany
István Molnár - Czech Academy of Sciences, Institute of Experimental Botany
Jaroslav Doležel - Czech Academy of Sciences, Institute of Experimental Botany
Eric R Ward - AgBiome
T Lynne Reuber - 2Blades Foundation
Jonathan D G Jones - Sainsbury Laboratory
Martin Mascher - Leibniz Institute of Plant Genetics and Crop Plant Research
Brian J Steffenson - University of Minnesota
Brande B H Wulff - King Abdullah University of Science and Technology
Publication Details: Nature communications, Vol.13(1), pp.1607-1607
Grant note: BBS/E/J/000PR9780 / Biotechnology and Biological Sciences Research Council
Identifiers: 9942251908331
Academic Unit: King Abdullah University of Science & Technology
Language: English
Resource Type: Journal article