Abstract
A novel bacterium designated strain B-16 was isolated from the compost leachate of the municipal solid waste (MSW) in a laboratory reactor. This strain was identified as a gram-negative bacterium,
Achromobacter xylosoxidans that could grow on bisphenol A (BPA, a representative endocrine disruptor) as a sole carbon source under aerobic condition. BPA-degrading characteristics of strain B-16 were investigated in liquid cultures. The results show that BPA degradation was influenced by several factors (e.g. inoculum size, substrate concentration, temperature and pH, etc). The half-lives, optimum temperature and pH were found to be 0.58–3.1
d, 35
°C and 7.0, respectively. BPA-degrading activity and cell growth were inhibited at high substrate concentration. Metabolic intermediates detected during the degradation process were identified as
p-hydroxybenzaldehyde,
p-hydroxybenzoic acid and
p-hydroquinone, respectively. Metabolic pathway of BPA degradation was proposed in this study.