Abstract
Teucrium royleanum was tested by four variant assays using lettuce seeds, showing excellent inhibition. The plants leaf leachates were assayed by Sandwich and Homogenated Sandwich Methods while Dish Pack Method was used for the evaluation of essential oils. The above stated methods confirm the presence of allelochemicals in T. royleanum and were further evaluated in bioassay guided way by total activity approach. The methanol subsequent fractions; hexane, chloroform, ethylacetate, butanol and water fractions were assayed by the Total Activity Method. The essential oils, hexane and chloroform fractions played a decisive role in our findings. The results from this study suggested that the secondary metabolites from T. royleanum can be potential candidates for the phytotoxicity (Allelopathy) and can be utilized on commercial scale for the development of new herbicides or weedicides.