Abstract
An amperometric cholesterol biosensor was fabricated by electropolymerization method of conducting polymers on pencil graphite electrode (PGE) after which apo-cholesterol oxidase (apo-ChOx) was immobilized onto a flavin adenine dinudeotide (FAD) monolayer by reconstitution method. In this study two well-known and widely used materials in the field of biosensors thiophene-3-boronic acid and 3-aminophenyl boronic acid were electropolymerized onto PGE and modified with cholesterol oxidase. Electrochemical performance of modified electrodes and their properties were compared. Based on the results, thiephene-3-boronic acid based electrode shows sensitivity 10 times higher response than that of one based on 3-aminophenyl boronic add. The effect of pH and temperature were assessed as well the reusability and stability of the fabricated biosensor were discussed. (C) 2016 Elsevier B.V. All rights reserved.